Keep the seeds moist, but not soaked, through the germination phase. Apply the seedling heat mat to the bottom of the tray and cover the top of the seed starting tray with a humidity trapping dome. Small RNA and degradome sequencing reveals important microRNA function in Astragalus chrysochlorus response to selenium stimuli. Place the seed into the -inch hole and cover gently with soil. Molecular chaperones and protein folding in plants. Pathogenesis-related proteins and peptides as promising tools for engineering plants with multiple stress tolerance. doi: 10.1007/s1201-9.Īli S, Ganai BA, Kamili AN, Bhat AA, Mir ZA, Bhat JA, Tyagi A, Islam ST, Mushtaq M, Yadav P, Rawat S, Grover A. Exogenous proline application reduces phytotoxic effects of selenium by minimising oxidative stress and improves growth in bean (Phaseolus vulgaris L.) seedlings.
Our data revealed that the responses of pepper to Se stress involve various pathways.ĭifferential expression protein Heat shock proteins Metabolic process Pepper Selenium stress.Īggarwal M, Sharma S, Kaur N, Pathania D, Bhandhari K, Kaushal N, Kaur R, Singh K, Srivastava A, Nayyar H. These results provide insights that may facilitate further studies on the pepper proteome expressed downstream of the Se stress response. Furthermore, several heat shock proteins were identified as DEPs. The DEPs were most strongly associated with the terms "metabolic process," "posttranslational modification, protein turnover, chaperones," and "protein processing in endoplasmic reticulum" according to Gene Ontology, eukaryotic orthologous groups classification, and Kyoto Encyclopedia of Genes and Genomes enrichment analysis, respectively. According to the above data, we performed a systematic bioinformatics analysis of all identified proteins and differentially expressed proteins (DEPs). Among them, the expression of 172 proteins was up-regulated, and the expression of 28 proteins was down-regulated in the Se/mock treatment comparison. A total of 4,693 proteins were identified, 3,938 of which yielded quantitative information. Here, to elucidate the effects of Se stress on the protein levels in pepper, we used an integrated approach involving tandem mass tag labeling, high performance liquid chromatography fractionation, and mass spectrometry-based analysis. To address the challenge of Se poisoning in plants, the mechanisms underlying Se-induced stress in plants must be understood.
Se fertilizer has been used to increase the Se content in crops to meet the Se requirements in humans and animals. Selenium (Se) is an essential trace element for human and animal health.
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